On modern farms, liquid nitrogen (LN2) tanks play a vital role in animal breeding and genetic preservation. They store frozen semen and embryos at a stable temperature of –196°C, ensuring long-term viability. However, to keep these tanks reliable, they must be refilled with liquid nitrogen regularly. The refilling process is both technical and safety-oriented.
The first step is pre-checking the tank condition. Farmers or technicians inspect the exterior of the tank for dents, frost buildup, or lid damage, as these could affect insulation and vacuum performance. Next, the liquid level inside the tank is measured using a calibrated dipstick or ruler. This determines how much nitrogen needs to be added.
Once preparation is complete, the refilling process begins. The technician carefully connects the transfer hose from the supply dewar to the farm storage tank. The transfer must be slow and controlled, as liquid nitrogen expands rapidly when exposed to air. During filling, a cloud of cold vapor rises from the tank—a normal sign of liquid entering. Operators always wear protective gloves and face shields to prevent frostbite, since LN2 can cause instant skin damage.
After reaching the proper level, the hose is removed, and the tank lid is quickly secured. The technician then rechecks the liquid level to confirm the refill amount. Finally, the tank is labeled with the date of refilling, ensuring proper maintenance records.
This entire process ensures that the farm’s genetic material remains safe and viable. Without timely refilling, nitrogen levels could drop, putting valuable livestock breeding resources at risk. Proper handling of LN2 tanks not only guarantees effective storage but also extends the lifespan of the equipment, making it a cornerstone of modern agricultural breeding practices.
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