Thawing Cup Cleaning and Disinfection Protocol: A Standardized Process to Prevent Cross-Contamination

In IVF and cryogenic laboratories, thawing cups play a critical role in safely warming embryos, cells, and biological samples. However, improper cleaning or disinfection can lead to cross-contamination, compromising sample integrity and clinical outcomes. To maintain a sterile environment, a standardized cleaning and disinfection protocol is essential.

1. Immediate Rinse After Use

Immediately after thawing, rinse the cup with distilled water to remove any biological residues. Avoid using tap water, which may introduce impurities or microbial contaminants.

2. Detergent-Based Cleaning

Use a non-toxic, lab-grade enzymatic detergent to break down protein and lipid residues. Clean the cup using soft brushes or ultrasonic cleaners to ensure all inner surfaces are reached without scratching.

• Soak: 10–15 minutes

• Scrub: Gentle circular motion

• Rinse: Three times with sterile distilled water

3. Disinfection and Sterilization

After cleaning, immerse the cup in a validated disinfectant, such as 70% ethanol, quaternary ammonium solution, or peracetic acid (per institutional guidelines).

For heat-resistant cups, autoclaving at 121°C for 15–20 minutes is recommended.

4. Drying and Storage

Air-dry the cup in a laminar flow hood or on a sterile surface. Store in a sealed, dust-free container or sterile pouch until the next use. Label storage containers with the cleaning date to ensure traceability.

5. Record-Keeping and Validation

Maintain cleaning logs for each cup, including user initials, date, and method used. Regularly validate the cleaning process through biological monitoring or ATP testing.

Conclusion

By following a standardized cleaning and disinfection protocol, labs can minimize contamination risk and ensure safe, repeatable thawing procedures—safeguarding sample quality and patient outcomes.